8/31/2023 0 Comments G force scale comparison![]() ![]() Centrate backpressure is applied to ensure hydrohermetic operation and that the centrifuge bowl remains full. Differences in density force cells to the periphery of the bowl, where they accumulate in the solids space, while cell-free centrate flows up the stacked discs through a centripetal pump and is collected for further processing. A feed stream is fed into the centrifuge bowl while it rotates at a high g-force. Disc-stack centrifuges are fed continuously but discharge solids either periodically or continuously ( 3). The latter are commonly used for clarification in the biopharmaceutical industry. Typical centrifuges used for such applications are tubular-bowl designs used in low volume processes, decanters used when feed-solids concentrations are high (e.g., in sewage water separations), and disc-stack centrifuges. GEA WESTFALIA (Centrifugation is used in a number of bioprocess applications, including clarification of solutions from hormone, vaccine, and enzyme production bacterial culture harvest and concentration and separation of blood fractions ( 3). Significant technological advances in centrifugation design and operating conditions minimized shear effects on mammalian cells ( 2). That shift to centrifugation was facilitated by the need to scale processes up to handle 10,000-L to 15,000-L bioreactors, with increasing harvest cell culture densities and titers. ![]() The following decade saw an industry-wide adoption of centrifugation with traditional periodic discharge of solids for primary clarification of mammalian cell cultures. Process-scale centrifugation was considered to be a significant capital investment, and bioprocessors had limited ability to control (minimizing) the effects of shear on mammalian cells. This process removes cells and cellular debris from the culture fluid to produce a clarified cell culture supernatant that will be suitable for further purification ( 1).īefore 2000, depth and tangential-flow microfiltration were standard clarification technologies in the biopharmaceutical industry. GEA WESTFALIA (Clarification is typically the first unit operation in the purification of monoclonal antibodies (MAbs) and other proteins from mammalian cell cultures. ![]()
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